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Molecular-Cloning
271.
ligation - very small insert - problems during ligation
(reply: 11)
272.
How to find DNA sequence upstream of gene? -
(reply: 3)
273.
Multimerization after restriction digestion -
(reply: 9)
274.
low concentartion of DNA after miniprep -
(reply: 6)
275.
pcr template amount -
(reply: 2)
276.
cloning kills -
(reply: 5)
277.
help. Is this double digest right? -
(reply: 5)
278.
TOPO TA Cloning - hundreds of colonies, 0 insert -
(reply: 6)
279.
Adding Anitbiotic to Cooled Agar Plates -
(reply: 8)
280.
cloning pGEMT -
(reply: 4)
281.
gel extraction -
(reply: 1)
282.
pcr template -
(reply: 4)
283.
how to find the intron size -
(reply: 2)
284.
how to design a northen blot probe? -
(reply: 1)
285.
Western blotting for Histones -
(reply: 1)
286.
how to clone the promoter of a gene -
(reply: 3)
287.
generating an array by gene amplification -
(reply: 2)
288.
genome amplification before cloning? -
(reply: 2)
289.
how to clone multiple inserts simulataneously - need to make a triple fusion
(reply: 1)
290.
Double digest and 3' end polishing with klenow -
(reply: 1)
291.
how to clone unstable DNA? -
(reply: 4)
292.
Which is the best way of determining the concentration of DNA? -
(reply: 3)
293.
PCR on template with trinucleotide repeats - PCR tips for complexe DNA template
(reply: 4)
294.
Ligation problem? -
(reply: 10)
295.
positive colony PCR, but no insert!? -
(reply: 5)
296.
how to do serial digestion with EcoR1 and bamH1 -
(reply: 5)
297.
No colonies -
(reply: 3)
298.
why OD600=0.3~0.4 is important? -
(reply: 8)
299.
Why does insert has two bands after RE digestion? -
(reply: 3)
300.
could anybody help me? -
(reply: 3)
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