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511. Problems with certain restriction enzymes - on the verge of tears - (reply: 10)
512. PPARs expression in cardiac cells - (reply: 4)
513. E.coli promoterless lacZ vector - (reply: 2)
514. EL250 relectroporation - (reply: 4)
515. klenow/T4 question - Making one end blunt (reply: 4)
516. PmeI-a mind of it's own? - (reply: 4)
517. preparation of electroporation competent cells from chemically competent cells - (reply: 3)
518. do the competen cells chang the U to T - (reply: 4)
519. plasmid degradation in tris EDTA - (reply: 10)
520. Cloning of construct in SalI site - (reply: 3)
521. Ligating with large insert - (reply: 5)
522. how many white colonies should i choose to analyze? - TA coloning problem (reply: 4)
523. DNA pellet won't go into solution - (reply: 4)
524. Product of ligation storage - (reply: 3)
525. Digestion question - (reply: 1)
526. PstI/SapI digestion problem - restriction enzyme (reply: 3)
527. Clonig Problem - (reply: 9)
528. opposite sequence orientation in TA cloning - (reply: 2)
529. MaxiPrep low plazmid concentration - (reply: 6)
530. Site-directed mutagenesis problem - Mutations under oligo (reply: 2)
531. Experience with Ultramers from IDT? - Long oligos (reply: 1)
532. NEED HELP-verification of my inserts - (reply: 13)
533. RNA extraction - How to take a pancreas sample and get RNA before degradation? (reply: 3)
534. multi fragment ligations - (reply: 2)
535. why should the conversion efficiency of competent cell be at least 10^8 cfu/_ - (reply: 2)
536. can i store transformed bacteria and for how long - (reply: 5)
537. QuikChange™ Site-Directed Mutagenesis Kit - (reply: 4)
538. random mutation during mutagenesis - will all colonies on LB plate have the same mutation? (reply: 3)
539. DNA yields with the Qiagen PCR gel purification kit - (reply: 8)
540. cassette pcr - (reply: 2)
512. PPARs expression in cardiac cells - (reply: 4)
513. E.coli promoterless lacZ vector - (reply: 2)
514. EL250 relectroporation - (reply: 4)
515. klenow/T4 question - Making one end blunt (reply: 4)
516. PmeI-a mind of it's own? - (reply: 4)
517. preparation of electroporation competent cells from chemically competent cells - (reply: 3)
518. do the competen cells chang the U to T - (reply: 4)
519. plasmid degradation in tris EDTA - (reply: 10)
520. Cloning of construct in SalI site - (reply: 3)
521. Ligating with large insert - (reply: 5)
522. how many white colonies should i choose to analyze? - TA coloning problem (reply: 4)
523. DNA pellet won't go into solution - (reply: 4)
524. Product of ligation storage - (reply: 3)
525. Digestion question - (reply: 1)
526. PstI/SapI digestion problem - restriction enzyme (reply: 3)
527. Clonig Problem - (reply: 9)
528. opposite sequence orientation in TA cloning - (reply: 2)
529. MaxiPrep low plazmid concentration - (reply: 6)
530. Site-directed mutagenesis problem - Mutations under oligo (reply: 2)
531. Experience with Ultramers from IDT? - Long oligos (reply: 1)
532. NEED HELP-verification of my inserts - (reply: 13)
533. RNA extraction - How to take a pancreas sample and get RNA before degradation? (reply: 3)
534. multi fragment ligations - (reply: 2)
535. why should the conversion efficiency of competent cell be at least 10^8 cfu/_ - (reply: 2)
536. can i store transformed bacteria and for how long - (reply: 5)
537. QuikChange™ Site-Directed Mutagenesis Kit - (reply: 4)
538. random mutation during mutagenesis - will all colonies on LB plate have the same mutation? (reply: 3)
539. DNA yields with the Qiagen PCR gel purification kit - (reply: 8)
540. cassette pcr - (reply: 2)