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871. cloning size - plasmid (reply: 2)
872. Help with Cloning into LARGE vector - (reply: 11)
873. double digestion with BamHI and HindIII - (reply: 13)
874. why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
875. How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
876. Help w/ CLONING...lots of transformants but no insert... - (reply: 8)
877. Eco47III is it problematic? - Selfligation doesnot work with Eco47III... (reply: 10)
878. volume of ligation product - (reply: 6)
879. plasmid stability - (reply: 4)
880. southern blot probe design - (reply: 2)
881. cloning large inserts in large vectors - (reply: 4)
882. 2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)
883. Strange result after ligation and transformation - (reply: 2)
884. klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble - (reply: 5)
885. cloning excised product in pGBKT7 doesn't work! - (reply: 3)
886. very easy sticky end ligation: no colonies! - Cloning masters, please help doomed cloner! (reply: 28)
887. Problems with T-DNA integration via Agrobacterium - (reply: 2)
888. how can i know if the sequence of my insert is toxic - (reply: 1)
889. Water and cloning? - Major cloning disfunction... (reply: 18)
890. Trouble with ligation - (reply: 7)
891. Genomic DNA PCR vs plasmid DNA PCR - (reply: 6)
892. pcr-product cloning problems - cloning of microsatellites (reply: 4)
893. Qiagen Kit Money Saver - (reply: 5)
894. TA cloning help - (reply: 11)
895. shorter vector fragment at the end of the cloning - (reply: 8)
896. Clone two pieces of inserts - (reply: 6)
897. Why is the fragment of insert shorter than I want? - (reply: 4)
898. How long for ligations? - (reply: 11)
899. useful tips for low melting temperature gel purification. - please share your experiences. (reply: 2)
900. blue light-sybr green - anybody see bands with sybr and blue light (reply: 3)
872. Help with Cloning into LARGE vector - (reply: 11)
873. double digestion with BamHI and HindIII - (reply: 13)
874. why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
875. How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
876. Help w/ CLONING...lots of transformants but no insert... - (reply: 8)
877. Eco47III is it problematic? - Selfligation doesnot work with Eco47III... (reply: 10)
878. volume of ligation product - (reply: 6)
879. plasmid stability - (reply: 4)
880. southern blot probe design - (reply: 2)
881. cloning large inserts in large vectors - (reply: 4)
882. 2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)
883. Strange result after ligation and transformation - (reply: 2)
884. klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble - (reply: 5)
885. cloning excised product in pGBKT7 doesn't work! - (reply: 3)
886. very easy sticky end ligation: no colonies! - Cloning masters, please help doomed cloner! (reply: 28)
887. Problems with T-DNA integration via Agrobacterium - (reply: 2)
888. how can i know if the sequence of my insert is toxic - (reply: 1)
889. Water and cloning? - Major cloning disfunction... (reply: 18)
890. Trouble with ligation - (reply: 7)
891. Genomic DNA PCR vs plasmid DNA PCR - (reply: 6)
892. pcr-product cloning problems - cloning of microsatellites (reply: 4)
893. Qiagen Kit Money Saver - (reply: 5)
894. TA cloning help - (reply: 11)
895. shorter vector fragment at the end of the cloning - (reply: 8)
896. Clone two pieces of inserts - (reply: 6)
897. Why is the fragment of insert shorter than I want? - (reply: 4)
898. How long for ligations? - (reply: 11)
899. useful tips for low melting temperature gel purification. - please share your experiences. (reply: 2)
900. blue light-sybr green - anybody see bands with sybr and blue light (reply: 3)