Residues don't match... why? - in human and mouse proteins (Jul/30/2008 )

Well, this is my doubt.

I want to do some site directed mutagenesis experiments with a membrane receptor in serines 920, 1068 and 1173 (published for human receptor). Those are the residues published in many papers... no problem with that. BUT when I look the aminoacid sequence of mouse receptor -which is the mutagenesis target- I can't found the same residues in THAT position. I mean, the residues I found are serines 917, 1071 and 1174 according to an alignment.

So, serines 920, 1068 and 1173 in human receptor are 917, 1071 and 1174 in mouse receptor? Why they're moved?? Can I assume that serines I want mutate are THE SAME in human and mouse proteins? If not.... what is wrong with this?

I hope help from somewhere....

Thanks.

Aleruiz

Hi

Its very likely that the mouse protein of interest will have 2 amino acid less than that of the human homologue. there can be variations in protein length between human & mouse homologues. Best thing would be to do an alignment of the protein sequences and check the conservation of the three serine residues of interest in mouse, human ( and other species as well, if available). Then you can proceed to mutate the three serines with great confidence.

Regards
Avinash

http://aviprem.gq.nu

Ok. Thanks a lot, Avinash!!! I'll follow your advice.

Nice website, btw.

Alex.