Blunting by Klenow - Excess dNTPs (Jul/29/2008 )
Would an excess of dNTPs affect a blunting reaction by Klenow
-Fledgeling-
QUOTE (Fledgeling @ Jul 30 2008, 04:33 AM)
Would an excess of dNTPs affect a blunting reaction by Klenow
i am not sure of klenow, but i used T4 DNA polymerase with excess dNTPs than recommended ..never had any problems with blunting.
cheers
-gene_tag-
QUOTE (Fledgeling @ Jul 30 2008, 07:03 AM)
Would an excess of dNTPs affect a blunting reaction by Klenow
No, i don't think so. I always use excess amount of dNTPs and everything with Klenow.
-yja97-