Transformation - thanks for help (Aug/10/2008 )

Dear Bioforumers:
Iam trying to clone scfv these days,actually my reagents are quite old.I did transformation yesterday and I got only white colonies and no blue ones.
should I do plasmid miniprep or suspect this result.
Thanks

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yes its always better to do the miniprep and check for the presence of DNA...considering that thr could be self ligated plasmid too..
-Divya

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How can I know that my IPTG and XGal working or not

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Do you have any of your vector left? Surely all you need to do is a vector ligation control on this sample and they should all be blue.

I always used to run a vector alone control in my cloning for this very reason (and sometimes an insert alone to check for any carry over of donor vector).

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Thanks Chris

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Thanks I did minipreps and RePCr pf plasmids the results were excellent

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Great news

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