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[32] phosphorylation hints needed! - (Jan/04/2006 )

Hi, I have just started to work with the phosphorylation [32P]. I have several questions & I would be deeply grateful if somebody would give me some hints. Should I always dry the gel? I cannot see any sharp bands just some dark pools? Does exposure of nitrocellulose to Kodak MR BioMax work, how long should I expose the film? I know that those are rather blunt and basic question however I would be grateful if somebody give me some advice. Best wish

-papryka2-

QUOTE (papryka2 @ Jan 4 2006, 02:57 PM)
Hi, I have just started to work with the phosphorylation [32P]. I have several questions & I would be deeply grateful if somebody would give me some hints. Should I always dry the gel? I cannot see any sharp bands just some dark pools? Does exposure of nitrocellulose to Kodak MR BioMax work, how long should I expose the film? I know that those are rather blunt and basic question however I would be grateful if somebody give me some advice. Best wish


What experiment are you doing?
Are you doing northern blot?

-Minnie Mouse-