double digestion - Time for digestion (Aug/31/2005 )

Please help. How long I need to keep for double digestion when I am using the enzymes NcoI and HindIII. Please help me in this regard

Is it possible for me to inactive these enzymes at 65C after digestion

Hi joey-

If you have not checked it out I always find the NEB webiste to be of great help. (www.neb.com)
Just click on the double digest link on their front page. Best of luck.

Kevin