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Cloning - Electrocompetent vs chemically competent (Aug/25/2005 )

Hi!

I want to clone a fragment that is 5-7kb long, and Iwonder if I should use electrocompetent cells or chemically competent? What is the difference between the methods?

-johku968-

hi,
chemically competent always worked fine for me, i've used it to clone upto 8.5 kb gene, or u may go for commercial supercompetent cells.
good luck
nbj

-nbj-