Cloning - Electrocompetent vs chemically competent (Aug/25/2005 )
Hi!
I want to clone a fragment that is 5-7kb long, and Iwonder if I should use electrocompetent cells or chemically competent? What is the difference between the methods?
-johku968-
hi,
chemically competent always worked fine for me, i've used it to clone upto 8.5 kb gene, or u may go for commercial supercompetent cells.
good luck
nbj
-nbj-