PQE vector - Getting protein in its soluble form (Sep/06/2005 )

I have cloned a protein ( N-terminus) in PQE vector and I want to get its soluble form to study its biological activities and its regulation pathway. I tried almost all possibilities to solubalise it but i was not able to get it in its native form and denatured form is not what i want . Please give me some tips.
Thanx in advance.

does this vector express a fusion protein (6His tag, Thioredoxin, etc)?

how well is the protein expressing?

I have cloned a protein ( N-terminus) in PQE vector and I want to get its soluble form to study its biological activities and its regulation pathway. I tried almost all possibilities to solubalise it but i was not able to get it in its native form and denatured form is not what i want     . Please give me some tips.
Thanx in advance.

when you say you've tried almost all possibilites do you mean growth and expression temperatures, IPTG concentrations, different cracking buffers?

if those don't work it all it's possible that the tag is interferring with the folding of the protein.....