Dilute re-ligation - deletion construct (Jun/06/2007 )
Hello,
I am about to delete an area of my gene using restriction digests. I am not using any inserts. My mentor told me to do a "dilute ligation" to recirculize the vector. Does anyone know how to do this
-tara123-
QUOTE (tara123 @ Jun 6 2007, 01:17 PM)
Hello,
I am about to delete an area of my gene using restriction digests. I am not using any inserts. My mentor told me to do a "dilute ligation" to recirculize the vector. Does anyone know how to do this
I am about to delete an area of my gene using restriction digests. I am not using any inserts. My mentor told me to do a "dilute ligation" to recirculize the vector. Does anyone know how to do this
to inhibit multimer formation ligate at 1-2ng/ul concentration...
-beccaf22-