RNA extraction - How to take a pancreas sample and get RNA before degradation? (Aug/28/2007 )

Hi everybody,
I'd like to know if any of you has experience extracting RNA from pancreas...I need to take the tissue sample and then do the RNA - DNA purification, I know it's pretty hard to do it since the pancreas in particular has lots of enzymes which can quickly degradate RNA before I can get the chance to extract it..

Do you know anything about what to do?? after killing the rat?
All I know so far is that dissection has to be as fast as possible, and I should perform the purification just after that..........I'm planning to use Trizol...

Well,
hope you can give me any suggestion

biotech!

Please....anybody!!

well i don't use to extract on whole tissue but i would cut with clean scalpel in tiny pieces and freeze in nitrogen. then crush it in RNA later or use automatic crusher with metal beads. Then trizol procedure.

Use RNALater.

http://www.ambion.com/catalog/CatNum.php?7020