QuikChange™ Site-Directed Mutagenesis Kit - (Aug/28/2007 )
i have a Question for PRIMER DESIGN in QuikChange™ Site-Directed
Mutagenesis Kit
when i want to insert 4 amino acid the really Tm should contain the 4 amino acid or not
and if any one has experience in this kit let tell us who he can use it
thank you
Mutagenesis Kit
when i want to insert 4 amino acid the really Tm should contain the 4 amino acid or not
and if any one has experience in this kit let tell us who he can use it
thank you
The Tm should only be calculated on the bases that will anneal to the template, so you shouldn't include the bases you are mutagenising.
Hi,
Stratagene has a nice website on site-directed mutagenesis. They also have an online oligo design program, which you may find useful. You'll have to register for an account (takes about 10 seconds) but will have access to their manuals.
http://stratagene.com/products/showCategory.aspx?catId=190
Good luck!
underprocess
Hello friends
I did what the say in the kit but I have a problem when I get my amplified pcr I digested with Dpn1 after that I transform in competent cells after that I can't have any colony in the petridish and I’m sure a bout my competent cell I did (+) control and I have the colony so I’m really surprise
Do any one had opinion for this situation