whats the deal with satellite colonies? - (Aug/27/2008 )

This might be a stupid and obvious question to most on this forum but I dont get why picking satellite colonies is so bad? I mean why is it more likely that the right plasmid is going to be in the main colony?

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Satellite colonies are non-transformed cells that grow in the shadow of transformed colonies (once the antibiotic in the medium has been broken down a little). So it's very likely that your plasmid will be in the 'main' colony

Clare

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