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ligation - (Jun/12/2006 )

hello
i want to ligate a dna fragment (289pb) to do a long fragment
I have the fragment with blunt ends and too with cohesive ends (SalI/XhoI)
which volume and unit of T4 dna ligase must i do ?
thanks very much

-romain30-

I do ligation in a total volume of 20ul, but there r people who do it in 10ul.
I use 1ul of T4 ligase (NEB).

-scolix-