Gene cloning - Gene library (Oct/27/2006 )

Hiya. I am not sure that is the "gene library" the same as "gene bank"? what is the multiple cloning site and if the restriction enzyme cut the sites why they cannot be expressed related to their small sizes? thx

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gene library: A collection of cloned DNA fragments which, taken together, represent the entire genome of a specific organism. Such libraries or "gene banks" are assembled so as to allow the isolation and study of individual genes. Gene libraries are produced by first breaking up or "fractionating" an entire genome. This fractionation can be accomplished either by physical methods or by use of restriction enzymes. The genome fragments are then cloned (multiplied in number) and stored for later use.
multiple cloning site: A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (usually 20+) restriction sites - a standard feature of engineered plasmids. It is thus used during procedures involving molecular cloning or subcloning. Extremely useful in biotechnology, bioengineering, and molecular genetics, MCSs let a biotechnologist insert a piece of DNA or several pieces of DNA into the region of the MCS. This can be used to create transgenic organisms, also known as genetically modified organisms (GMOs).

Try Wikipedia or a search engine next time. You can retrieve a lot of answers just by spending a little time.

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