Question about Nox gene - (Nov/17/2005 )

Dear all,
I am looking for a procedure to isolate effectively a target gene from a genome, for instance Nox gene from S.mutans. Could u please tell me the way.
One more question that i really wanna know about gateway system.
Thanks a lot,
yours scincerely,
satokaiba

I wanna support some more informations for my last topic that:
I did design the primers for that gene and used NcoI and PstI as the restriction site for it. However, i still wanna have a procedure to do experiments in the lab. Please help:-)
NOX gene is NADH oxidase gene and S.mutans is Streptococcus mutans.