how to get cosmid DNA back to E.coli? - (Aug/07/2006 )

Hi,
I need to know if it is possible to transform E.coli with cosmid vector DNA (approx 30 kb)?
If anyone has a protocol or a suggestion please help!

Puma

Hi,
Yeah you could use the simple protocl for transformation like one given in manniatis involving cacl2 treated competent cells. I have used this method for transforming 21 kb cosmid. I hope this helps.
uhsna




quote name='puma' date='Aug 7 2006, 12:27 PM' post='62861']
Hi,
I need to know if it is possible to transform E.coli with cosmid vector DNA (approx 30 kb)?
If anyone has a protocol or a suggestion please help!

Puma
[/quote]

You could also take advantage of the fact that it's a cosmid, and use a lambda packaging kit...