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could anybody help me? - (Apr/04/2008 )

Hi, all,

Can anyone tell me which vectors i can use to express proteins in S. cerevisia. I need a vector with a weak promoter and low copy number. I also want one vector with a strong promoter and high copy number to express my protein constitutively. It is OK if the vector has an inducible promoter. I searched these vectors online for long time, but i still have not found the ideal vectors.

Any suggestions will be greatly appreciated.

Thanks in advance!

-yuer-

Could anybody give me any suggestions? I really need to find these vectors within few days! Pleeeeezzzzzzzzzzzz!

-yuer-

QUOTE (yuer @ Apr 5 2008, 12:54 PM)
Could anybody give me any suggestions? I really need to find these vectors within few days! Pleeeeezzzzzzzzzzzz!


There aren't many people online at the moment - you'll just have to be a bit patient wink.gif

-Clare-

Hi, all,

Finally, i found the vectors I need from ATCC catalogue online. I still write the way the found the vectors just in case that some others need to find out vector to use. It is really a good way to look for vector from ATCC catalogue online. They have the vectors for different species and for different markers.

Thanks

-yuer-