How can I make a point mutation - (Sep/11/2006 )

I PCR amplified a gene for sub cloning, introducing 2 restriction sites at beginning and the end of the gene. I have to clone this gene into the hydrophilic loop of another gene. The prblem is there is one additional thymine in the beginning of the amplified gene to be cloned and it changes all the reading frame.
Is there any possibility to remove only this thymine, I mean any kind of mutation only to one base.

Kindly help, I am under pressure

You can do a quick change mutagenesis

Go to the Stratagene website. The kit is called Quikchange site directed mutagenesis and provides you with everything you need and is very good -Ive done several mutations now.

It will tell you how to design your primers for the mutation (which are basically the mutation in the middle with between 12 and 24 correct bases on either side for a single point mutation.)