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301. Expansion of commercial competent bacterial cell lines? - (reply: 2)
302. Manipulation of target DNA fragments - (reply: 1)
303. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
304. Appearance of a salty plasmid preparation - (reply: 1)
305. origami bacteria transformation - (reply: 5)
306. Smallest mRNA length that can be translated - (reply: 3)
307. wrong insert? right insert?? - (reply: 2)
308. Help~ Incorrect constructs in cloning - (reply: 3)
309. Problems with ligation and transformation - (reply: 2)
310. Transformation of Restricted plasmid - (reply: 3)
311. Scrambled sequence? - (reply: 3)
312. Why is my insert missing from my plasmid? - (reply: 4)
313. PCR Profile for ligation - (reply: 3)
314. Blunt end is ligating to sticky end? - (reply: 2)
315. SNP in the promoter region - (reply: 1)
316. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
317. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
318. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
319. New ordered Restriction Enzymes not working, need help! - (reply: 2)
320. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
321. Transfection but no expression - (reply: 4)
322. Change nucleotide - (reply: 1)
323. Colonies grown without plasmid - (reply: 7)
324. unwanted band - (reply: 5)
325. Colony PCR positive and Digestion negative????? - (reply: 11)
326. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
327. Plasmid on blotting paper - (reply: 1)
328. Dimerization of PCR product - (reply: 4)
329. How to generate Lentivirus? - (reply: 4)
330. Do genes always need to be cloned in the "right direction"? - (reply: 3)
302. Manipulation of target DNA fragments - (reply: 1)
303. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
304. Appearance of a salty plasmid preparation - (reply: 1)
305. origami bacteria transformation - (reply: 5)
306. Smallest mRNA length that can be translated - (reply: 3)
307. wrong insert? right insert?? - (reply: 2)
308. Help~ Incorrect constructs in cloning - (reply: 3)
309. Problems with ligation and transformation - (reply: 2)
310. Transformation of Restricted plasmid - (reply: 3)
311. Scrambled sequence? - (reply: 3)
312. Why is my insert missing from my plasmid? - (reply: 4)
313. PCR Profile for ligation - (reply: 3)
314. Blunt end is ligating to sticky end? - (reply: 2)
315. SNP in the promoter region - (reply: 1)
316. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
317. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
318. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
319. New ordered Restriction Enzymes not working, need help! - (reply: 2)
320. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
321. Transfection but no expression - (reply: 4)
322. Change nucleotide - (reply: 1)
323. Colonies grown without plasmid - (reply: 7)
324. unwanted band - (reply: 5)
325. Colony PCR positive and Digestion negative????? - (reply: 11)
326. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
327. Plasmid on blotting paper - (reply: 1)
328. Dimerization of PCR product - (reply: 4)
329. How to generate Lentivirus? - (reply: 4)
330. Do genes always need to be cloned in the "right direction"? - (reply: 3)