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181. Genomic ß-Lactamase? Blue White screening not working - (reply: 1)
182. direction of the expression units in multi-gene vectors - (reply: 1)
183. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
184. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
185. Measuring pcr fragments in a gel - (reply: 1)
186. gel electrophoresis - (reply: 4)
187. three questions about CRISPR knowout - (reply: 2)
188. Help needed for Ligation/Transformation - (reply: 1)
189. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
190. ClaI restriction enzyme efficency - (reply: 1)
191. Blunt end cloning - (reply: 12)
192. sequantial digest - (reply: 25)
193. Temperature sensitivity of Plasmids - (reply: 1)
194. DMSO in restriction digest - (reply: 1)
195. Molecular cloning - (reply: 5)
196. Nuclease free water for cloning - (reply: 8)
197. Directional cloning 2bps overhang - (reply: 3)
198. I need help designing homology arms for CRISPR - (reply: 3)
199. Partial digest XhoI NotI NEB - (reply: 6)
200. removing part from plasmid - (reply: 18)
201. Start codon after promoter - (reply: 1)
202. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
203. Purpose of PolyA in cloning - (reply: 1)
204. Ligation failure? I ran some on a gel - (reply: 6)
205. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
206. Cloning into pVIVO2 vector - (reply: 8)
207. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
208. Cloning worked, but the expression vector is weird - (reply: 6)
209. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
210. site directed mutagenesis - (reply: 3)
182. direction of the expression units in multi-gene vectors - (reply: 1)
183. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
184. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
185. Measuring pcr fragments in a gel - (reply: 1)
186. gel electrophoresis - (reply: 4)
187. three questions about CRISPR knowout - (reply: 2)
188. Help needed for Ligation/Transformation - (reply: 1)
189. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
190. ClaI restriction enzyme efficency - (reply: 1)
191. Blunt end cloning - (reply: 12)
192. sequantial digest - (reply: 25)
193. Temperature sensitivity of Plasmids - (reply: 1)
194. DMSO in restriction digest - (reply: 1)
195. Molecular cloning - (reply: 5)
196. Nuclease free water for cloning - (reply: 8)
197. Directional cloning 2bps overhang - (reply: 3)
198. I need help designing homology arms for CRISPR - (reply: 3)
199. Partial digest XhoI NotI NEB - (reply: 6)
200. removing part from plasmid - (reply: 18)
201. Start codon after promoter - (reply: 1)
202. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
203. Purpose of PolyA in cloning - (reply: 1)
204. Ligation failure? I ran some on a gel - (reply: 6)
205. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
206. Cloning into pVIVO2 vector - (reply: 8)
207. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
208. Cloning worked, but the expression vector is weird - (reply: 6)
209. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
210. site directed mutagenesis - (reply: 3)