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991. ligating small inert into a large vector - (reply: 4)
992. tranformation trouble using dh5alpha - (reply: 1)
993. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
994. Starting cells for making one's own competent cells - (reply: 7)
995. 6 months... and still no positive clones! - (reply: 4)
996. Really strange problem with sequencing and plasmid purification! - (reply: 8)
997. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
998. what is site-specific integration - (reply: 2)
999. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
1000. double transformations - (reply: 1)
1001. Unusual band in transformants - (reply: 2)
1002. ligation--pls help! - (reply: 10)
1003. bizarre digestion:) - (reply: 2)
1004. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
1005. metagenomic library consturction - BAC vectors (reply: 1)
1006. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
1007. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
1008. Chromosomal recombination with BL21? - (reply: 2)
1009. Restriction Digestion!! - (reply: 5)
1010. digestion - restriction enzymes (reply: 2)
1011. How to prevent self ligation - selfligation (reply: 11)
1012. what is the difference between DH10B and Top10 - (reply: 1)
1013. problems encountered when working on a large plasmid - (reply: 2)
1014. make a Mega plasmid mutation - (reply: 1)
1015. Cloning - (reply: 1)
1016. Copy-number calculation formula? - (reply: 1)
1017. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
1018. low melt agarose - (reply: 1)
1019. restriction digest - smear - (reply: 1)
1020. designing of primers - (reply: 2)
992. tranformation trouble using dh5alpha - (reply: 1)
993. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
994. Starting cells for making one's own competent cells - (reply: 7)
995. 6 months... and still no positive clones! - (reply: 4)
996. Really strange problem with sequencing and plasmid purification! - (reply: 8)
997. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
998. what is site-specific integration - (reply: 2)
999. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
1000. double transformations - (reply: 1)
1001. Unusual band in transformants - (reply: 2)
1002. ligation--pls help! - (reply: 10)
1003. bizarre digestion:) - (reply: 2)
1004. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
1005. metagenomic library consturction - BAC vectors (reply: 1)
1006. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
1007. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
1008. Chromosomal recombination with BL21? - (reply: 2)
1009. Restriction Digestion!! - (reply: 5)
1010. digestion - restriction enzymes (reply: 2)
1011. How to prevent self ligation - selfligation (reply: 11)
1012. what is the difference between DH10B and Top10 - (reply: 1)
1013. problems encountered when working on a large plasmid - (reply: 2)
1014. make a Mega plasmid mutation - (reply: 1)
1015. Cloning - (reply: 1)
1016. Copy-number calculation formula? - (reply: 1)
1017. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
1018. low melt agarose - (reply: 1)
1019. restriction digest - smear - (reply: 1)
1020. designing of primers - (reply: 2)