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1081. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1082. Dephosphorylation of vector - (reply: 8)
1083. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1084. rna isolation - (reply: 1)
1085. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1086. So many colonies! - (reply: 8)
1087. Large vector cloning-recombination problem? - (reply: 1)
1088. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1089. selecting recombinants - (reply: 1)
1090. colony PCR for subcloning gene - (reply: 1)
1091. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1092. cloning of a 20 kb insert - (reply: 2)
1093. DNA quantification after gel purification - (reply: 6)
1094. cloning of an operon - (reply: 2)
1095. storing plasmid - -80degrees (reply: 4)
1096. verifying a positive transformant - (reply: 3)
1097. Need advice on cloning very small insert into a vector - (reply: 3)
1098. Cloning problems i.e. Ligation - (reply: 2)
1099. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1100. Radioactive carbon - (reply: 3)
1101. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1102. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1103. Digesting large amount of plasmid - (reply: 4)
1104. cleanup of digestion before ligation - (reply: 6)
1105. transformation efficiency - (reply: 7)
1106. transformed bacteria doesnt grow - (reply: 1)
1107. TOPO Cloning - (reply: 5)
1108. blunt ligation - (reply: 4)
1109. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1110. About Laminin 5'UTR 's function - (reply: 1)
1082. Dephosphorylation of vector - (reply: 8)
1083. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1084. rna isolation - (reply: 1)
1085. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1086. So many colonies! - (reply: 8)
1087. Large vector cloning-recombination problem? - (reply: 1)
1088. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1089. selecting recombinants - (reply: 1)
1090. colony PCR for subcloning gene - (reply: 1)
1091. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1092. cloning of a 20 kb insert - (reply: 2)
1093. DNA quantification after gel purification - (reply: 6)
1094. cloning of an operon - (reply: 2)
1095. storing plasmid - -80degrees (reply: 4)
1096. verifying a positive transformant - (reply: 3)
1097. Need advice on cloning very small insert into a vector - (reply: 3)
1098. Cloning problems i.e. Ligation - (reply: 2)
1099. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1100. Radioactive carbon - (reply: 3)
1101. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1102. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1103. Digesting large amount of plasmid - (reply: 4)
1104. cleanup of digestion before ligation - (reply: 6)
1105. transformation efficiency - (reply: 7)
1106. transformed bacteria doesnt grow - (reply: 1)
1107. TOPO Cloning - (reply: 5)
1108. blunt ligation - (reply: 4)
1109. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1110. About Laminin 5'UTR 's function - (reply: 1)