|
571. why do some colonies keep growing in the chiller? - (reply: 7)
572. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
573. Difference between types of bacterial culture broths? - (reply: 4)
574. Co-transfection with 2 plasmids - (reply: 10)
575. Low passage HEK293?? - (reply: 3)
576. Vector Digestion Problem - (reply: 4)
577. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
578. Transformation with a big plasmid - (reply: 4)
579. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
580. Three way ligation problem - (reply: 6)
581. problems with recovery of dna from gel and ligation - (reply: 5)
582. Colony PCR and digestion with KpnI - (reply: 10)
583. Greenish tinge to bacterial culture - (reply: 3)
584. SURE E. coli competent cells - (reply: 1)
585. Ligation - (reply: 20)
586. E. coli growing on plates and bot not in LB medium - (reply: 10)
587. *** Human Insulin promoter sequence*** - (reply: 2)
588. digested plasmid - (reply: 2)
589. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
590. Subcloning - RE digest and ligation and transformation - (reply: 5)
591. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
592. Making DNA Linkers for cloning purposes - (reply: 1)
593. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
594. Dam-/+ e. coli strains - (reply: 6)
595. cloning short genomic DNA fragments - (reply: 1)
596. finding the protein and nucleotide matches - (reply: 1)
597. DH10B with big construct question - (reply: 2)
598. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
599. cloning to pET28a - (reply: 1)
600. Weird Library Transformation Results - (reply: 10)
572. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
573. Difference between types of bacterial culture broths? - (reply: 4)
574. Co-transfection with 2 plasmids - (reply: 10)
575. Low passage HEK293?? - (reply: 3)
576. Vector Digestion Problem - (reply: 4)
577. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
578. Transformation with a big plasmid - (reply: 4)
579. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
580. Three way ligation problem - (reply: 6)
581. problems with recovery of dna from gel and ligation - (reply: 5)
582. Colony PCR and digestion with KpnI - (reply: 10)
583. Greenish tinge to bacterial culture - (reply: 3)
584. SURE E. coli competent cells - (reply: 1)
585. Ligation - (reply: 20)
586. E. coli growing on plates and bot not in LB medium - (reply: 10)
587. *** Human Insulin promoter sequence*** - (reply: 2)
588. digested plasmid - (reply: 2)
589. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
590. Subcloning - RE digest and ligation and transformation - (reply: 5)
591. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
592. Making DNA Linkers for cloning purposes - (reply: 1)
593. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
594. Dam-/+ e. coli strains - (reply: 6)
595. cloning short genomic DNA fragments - (reply: 1)
596. finding the protein and nucleotide matches - (reply: 1)
597. DH10B with big construct question - (reply: 2)
598. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
599. cloning to pET28a - (reply: 1)
600. Weird Library Transformation Results - (reply: 10)