Recovery of competence of BH10 bacteria - is it possible? - (Oct/24/2013 )

Hi all,

Do you know whether competency of BH10 bacteria could be recovered chemically after unexpected thawing following refrigerator break? Should I try to recovery them or just throw out? Are there any methods in case of it to recover bacteria's competence? Did someone have similar problem?

Thank you in advance for any advice

So long as you have some living bacteria, there are a number of methods for making competent cells.  These methods do not require the cells to be competent beforehand.

My favourite is: http://openwetware.org/wiki/TOP10_chemically_competent_cells (written up by Phage434), though it can be variable depending on the cell strain that you use.

Thank you for your advice! I really appreciate your support!