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271. GFP vector - (reply: 1)
272. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
273. no colony - (reply: 3)
274. Cloning beginner and clueless! - (reply: 1)
275. Which epitope tag to use for ChIP? - (reply: 1)
276. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
277. Ligation-transformation...Never faced such problem ever - (reply: 6)
278. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)
279. Can multiple genes follow only one promoter? - (reply: 1)
280. Restriction Digest Question - (reply: 4)
281. Problem with Large vector & very small insert - (reply: 1)
282. Storage life of plasmid DNA in water - (reply: 5)
283. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
284. Site-directed mutagenesis failed - (reply: 20)
285. Insert & vector too dilute to perform ligation - (reply: 3)
286. Cloning Problems - (reply: 2)
287. Suitable templates for in vitro transcription - (reply: 1)
288. Different ways to ensure the successful cloning? - (reply: 2)
289. Plasmid DNA digestion problem - (reply: 3)
290. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
291. Single codon insert - (reply: 3)
292. cloning- ligation - (reply: 3)
293. Sticky and blunt ligation issue - (reply: 6)
294. Handling DEPC under nitrogen/argon - (reply: 3)
295. Incubation period after heat shock at transformation--reason? - (reply: 3)
296. PCR product sequencing - (reply: 3)
297. What is the optimal time for transfection efficiency measuring? - (reply: 1)
298. i couldn't get the band of target gene after ligation - (reply: 9)
299. Minimum homology bases needed for homologous recombination - (reply: 1)
300. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
272. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
273. no colony - (reply: 3)
274. Cloning beginner and clueless! - (reply: 1)
275. Which epitope tag to use for ChIP? - (reply: 1)
276. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
277. Ligation-transformation...Never faced such problem ever - (reply: 6)
278. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)
279. Can multiple genes follow only one promoter? - (reply: 1)
280. Restriction Digest Question - (reply: 4)
281. Problem with Large vector & very small insert - (reply: 1)
282. Storage life of plasmid DNA in water - (reply: 5)
283. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
284. Site-directed mutagenesis failed - (reply: 20)
285. Insert & vector too dilute to perform ligation - (reply: 3)
286. Cloning Problems - (reply: 2)
287. Suitable templates for in vitro transcription - (reply: 1)
288. Different ways to ensure the successful cloning? - (reply: 2)
289. Plasmid DNA digestion problem - (reply: 3)
290. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
291. Single codon insert - (reply: 3)
292. cloning- ligation - (reply: 3)
293. Sticky and blunt ligation issue - (reply: 6)
294. Handling DEPC under nitrogen/argon - (reply: 3)
295. Incubation period after heat shock at transformation--reason? - (reply: 3)
296. PCR product sequencing - (reply: 3)
297. What is the optimal time for transfection efficiency measuring? - (reply: 1)
298. i couldn't get the band of target gene after ligation - (reply: 9)
299. Minimum homology bases needed for homologous recombination - (reply: 1)
300. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)