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841. Transformation - Transformation (reply: 1)
842. how to add 3XHA to the N-terminal of a protein - (reply: 5)
843. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
844. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
845. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
846. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
847. competent cells stock - (reply: 3)
848. Questions about pIRES-hrGFP1a - (reply: 1)
849. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
850. Cloning into lentiviral vector - (reply: 11)
851. cloning failure help plzzz - cloning (reply: 1)
852. use of pGEM -tT easy vectors - (reply: 2)
853. ~200kb Plasmid DNA purification is not working - (reply: 1)
854. ligating a 6 His tag to plasmid - (reply: 2)
855. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
856. ligation and transformation problem - (reply: 1)
857. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
858. Mini prep of frozen bacteria cells - (reply: 2)
859. PCR cloning problem - could not get colony (reply: 4)
860. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
861. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
862. PCR clonning - (reply: 3)
863. 2 bands in blue colony PCR - (reply: 5)
864. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
865. Introduction to vector development - Basics on vectors types and construction (reply: 1)
866. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
867. Targeted mutagenesis using a linear fragment - (reply: 1)
868. Transformation & Minimal Plates - (reply: 5)
869. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
870. Subcloning Problem - (reply: 3)
842. how to add 3XHA to the N-terminal of a protein - (reply: 5)
843. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
844. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
845. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
846. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
847. competent cells stock - (reply: 3)
848. Questions about pIRES-hrGFP1a - (reply: 1)
849. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
850. Cloning into lentiviral vector - (reply: 11)
851. cloning failure help plzzz - cloning (reply: 1)
852. use of pGEM -tT easy vectors - (reply: 2)
853. ~200kb Plasmid DNA purification is not working - (reply: 1)
854. ligating a 6 His tag to plasmid - (reply: 2)
855. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
856. ligation and transformation problem - (reply: 1)
857. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
858. Mini prep of frozen bacteria cells - (reply: 2)
859. PCR cloning problem - could not get colony (reply: 4)
860. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
861. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
862. PCR clonning - (reply: 3)
863. 2 bands in blue colony PCR - (reply: 5)
864. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
865. Introduction to vector development - Basics on vectors types and construction (reply: 1)
866. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
867. Targeted mutagenesis using a linear fragment - (reply: 1)
868. Transformation & Minimal Plates - (reply: 5)
869. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
870. Subcloning Problem - (reply: 3)