|
1321. Help: incompatible RE site subcloning - (reply: 1)
1322. need help cloning cdna of a known protein - (reply: 1)
1323. restriction problem - (reply: 3)
1324. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1325. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1326. Trouble with subcloning - (reply: 6)
1327. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1328. Lb-kan plates life - How long can they be used? (reply: 2)
1329. LR reaction - Cloning into a expression vector (reply: 1)
1330. TOPO-Transformation Problem - (reply: 4)
1331. Checking Plasmid Expression - (reply: 1)
1332. designing primers for genes not sequenced yet - (reply: 2)
1333. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1334. Cloning Problem - (reply: 3)
1335. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1336. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1337. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1338. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1339. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1340. Restriction buffer composition - (reply: 2)
1341. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1342. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1343. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1344. Yet another ligation problem - (reply: 4)
1345. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1346. Promoter Cloning for Luciferase Assay - (reply: 3)
1347. Creating mutants - (reply: 3)
1348. cloning protocol - (reply: 1)
1349. Ligation Time and Temp - (reply: 4)
1350. Miniprep - (reply: 3)
1322. need help cloning cdna of a known protein - (reply: 1)
1323. restriction problem - (reply: 3)
1324. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1325. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1326. Trouble with subcloning - (reply: 6)
1327. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1328. Lb-kan plates life - How long can they be used? (reply: 2)
1329. LR reaction - Cloning into a expression vector (reply: 1)
1330. TOPO-Transformation Problem - (reply: 4)
1331. Checking Plasmid Expression - (reply: 1)
1332. designing primers for genes not sequenced yet - (reply: 2)
1333. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1334. Cloning Problem - (reply: 3)
1335. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1336. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1337. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1338. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1339. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1340. Restriction buffer composition - (reply: 2)
1341. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1342. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1343. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1344. Yet another ligation problem - (reply: 4)
1345. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1346. Promoter Cloning for Luciferase Assay - (reply: 3)
1347. Creating mutants - (reply: 3)
1348. cloning protocol - (reply: 1)
1349. Ligation Time and Temp - (reply: 4)
1350. Miniprep - (reply: 3)