|
1441. problem in gene cloning - (reply: 4)
1442. Odd point mutations - (reply: 23)
1443. Plasmid linearization by PCR - (reply: 4)
1444. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1445. can't make the cloning work - (reply: 3)
1446. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1447. cloning help - (reply: 9)
1448. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1449. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1450. Ligation trouble continues - subcloning ligation failure (reply: 9)
1451. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1452. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1453. colony PCR - (reply: 8)
1454. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1455. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1456. Truncated sequence - (reply: 1)
1457. gateway cloning - (reply: 3)
1458. Purifying and cloning 69nt product - (reply: 5)
1459. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
1460. ligatin issues - (reply: 1)
1461. Chloramphenicol concentration in M9 minimal media - What is maniatis and where can I find it? lol (reply: 1)
1462. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1463. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
1464. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1465. A gateway cloning expression vector question - (reply: 1)
1466. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1467. Promoter/gene cloning question - (reply: 7)
1468. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1469. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
1470. vivo expression technology - ivet (reply: 25)
1442. Odd point mutations - (reply: 23)
1443. Plasmid linearization by PCR - (reply: 4)
1444. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1445. can't make the cloning work - (reply: 3)
1446. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1447. cloning help - (reply: 9)
1448. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1449. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1450. Ligation trouble continues - subcloning ligation failure (reply: 9)
1451. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1452. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1453. colony PCR - (reply: 8)
1454. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1455. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1456. Truncated sequence - (reply: 1)
1457. gateway cloning - (reply: 3)
1458. Purifying and cloning 69nt product - (reply: 5)
1459. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
1460. ligatin issues - (reply: 1)
1461. Chloramphenicol concentration in M9 minimal media - What is maniatis and where can I find it? lol (reply: 1)
1462. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1463. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
1464. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1465. A gateway cloning expression vector question - (reply: 1)
1466. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1467. Promoter/gene cloning question - (reply: 7)
1468. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1469. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
1470. vivo expression technology - ivet (reply: 25)