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601. Injecting mice with lentivirus - (reply: 1)
602. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
603. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
604. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
605. Ligation into a BAC? - (reply: 2)
606. oligo continually appears less on gel compared to other same load - (reply: 2)
607. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
608. plasmid tetR and tet promoter - (reply: 2)
609. unable to amplify an infectious retrovirus clone - (reply: 4)
610. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
611. No bands at all after double digestion - (reply: 1)
612. Digest for insert orientation - (reply: 4)
613. Colonies from transformation plate are not growing...:( - (reply: 5)
614. Problem with ligating a small insert (LoxP site) - (reply: 5)
615. Is the genomic DNA similar among different cell lines? - (reply: 4)
616. molecular cloning - (reply: 3)
617. Swich selection in plasmid - (reply: 3)
618. Sub-cloning and transformation - (reply: 17)
619. cloning without phosphorylation - (reply: 7)
620. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
621. A good vector to make gene library? - (reply: 5)
622. problems with midiprep - (reply: 3)
623. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
624. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
625. 900bp insertion into PIRESnEO,problem - (reply: 2)
626. fusion of cDNA expressing protein with gfp - (reply: 1)
627. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
628. Problem with plasmids transformation - (reply: 6)
629. Cloning genetic sequences of proteins. - (reply: 1)
630. problem of restriction enzyme digestion - (reply: 3)
602. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
603. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
604. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
605. Ligation into a BAC? - (reply: 2)
606. oligo continually appears less on gel compared to other same load - (reply: 2)
607. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
608. plasmid tetR and tet promoter - (reply: 2)
609. unable to amplify an infectious retrovirus clone - (reply: 4)
610. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
611. No bands at all after double digestion - (reply: 1)
612. Digest for insert orientation - (reply: 4)
613. Colonies from transformation plate are not growing...:( - (reply: 5)
614. Problem with ligating a small insert (LoxP site) - (reply: 5)
615. Is the genomic DNA similar among different cell lines? - (reply: 4)
616. molecular cloning - (reply: 3)
617. Swich selection in plasmid - (reply: 3)
618. Sub-cloning and transformation - (reply: 17)
619. cloning without phosphorylation - (reply: 7)
620. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
621. A good vector to make gene library? - (reply: 5)
622. problems with midiprep - (reply: 3)
623. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
624. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
625. 900bp insertion into PIRESnEO,problem - (reply: 2)
626. fusion of cDNA expressing protein with gfp - (reply: 1)
627. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
628. Problem with plasmids transformation - (reply: 6)
629. Cloning genetic sequences of proteins. - (reply: 1)
630. problem of restriction enzyme digestion - (reply: 3)