|
1411. Restriction digestion troubleshooting - (reply: 10)
1412. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1413. Which digestion enzyme? - (reply: 1)
1414. Cloning big fragment into smaller vector - (reply: 3)
1415. from where can i get the P1 phage - (reply: 1)
1416. multi way ligation or long range pcr? - (reply: 1)
1417. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1418. list of sequence to be added before RE site - (reply: 3)
1419. plasmid, BAC, or cosmid? - (reply: 3)
1420. Wierd trouble - dunno where my insert is?? (reply: 3)
1421. Large Molecular weight DNA Extarction - (reply: 7)
1422. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1423. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1424. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1425. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1426. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1427. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1428. Vector sequence included in (PCR) amplified insert - (reply: 2)
1429. COMPETENT CELL PREPARATION - (reply: 6)
1430. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1431. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1432. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1433. T A cloning problems - (reply: 3)
1434. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1435. Maximum recovery time after electroporation - (reply: 2)
1436. pCLBABE puro retriviral vector - (reply: 1)
1437. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1438. Blunt-end cloning problem - (reply: 6)
1439. Supercoiling blocking restriction site? - (reply: 6)
1440. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1412. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1413. Which digestion enzyme? - (reply: 1)
1414. Cloning big fragment into smaller vector - (reply: 3)
1415. from where can i get the P1 phage - (reply: 1)
1416. multi way ligation or long range pcr? - (reply: 1)
1417. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1418. list of sequence to be added before RE site - (reply: 3)
1419. plasmid, BAC, or cosmid? - (reply: 3)
1420. Wierd trouble - dunno where my insert is?? (reply: 3)
1421. Large Molecular weight DNA Extarction - (reply: 7)
1422. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1423. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1424. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1425. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1426. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1427. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1428. Vector sequence included in (PCR) amplified insert - (reply: 2)
1429. COMPETENT CELL PREPARATION - (reply: 6)
1430. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1431. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1432. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1433. T A cloning problems - (reply: 3)
1434. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1435. Maximum recovery time after electroporation - (reply: 2)
1436. pCLBABE puro retriviral vector - (reply: 1)
1437. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1438. Blunt-end cloning problem - (reply: 6)
1439. Supercoiling blocking restriction site? - (reply: 6)
1440. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)