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1201. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1202. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1203. BstUI digestion - (reply: 1)
1204. I have cloning problems with pGEM-T Easy - (reply: 5)
1205. CaCl2 for making competent cells - (reply: 3)
1206. best way to transport bacteria at room temperature - (reply: 5)
1207. Suicide vector - (reply: 2)
1208. Extremely small bacterial colonies after transformation - (reply: 10)
1209. tranformation efficiency - (reply: 2)
1210. transfection time - (reply: 2)
1211. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1212. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1213. Problem with SmaI restriction digest - (reply: 6)
1214. blunt-end / 3ŽA overhang cloning - (reply: 1)
1215. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1216. overexpression of stable clones - (reply: 1)
1217. functionality of yeast promoter in E. coli - (reply: 1)
1218. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1219. Can't seem to do a simple cloning - (reply: 2)
1220. Histogram from Nanodrop - (reply: 1)
1221. cDNA library construction - I get only one colony (reply: 2)
1222. Cloning into pIBV5-His (not working) - (reply: 1)
1223. Addition of Restriction sites into PCR primers - (reply: 4)
1224. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1225. Streaky DNA digest - (reply: 3)
1226. TOPO TA cloning problem - (reply: 12)
1227. cloning problem - (reply: 2)
1228. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1229. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1230. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1202. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1203. BstUI digestion - (reply: 1)
1204. I have cloning problems with pGEM-T Easy - (reply: 5)
1205. CaCl2 for making competent cells - (reply: 3)
1206. best way to transport bacteria at room temperature - (reply: 5)
1207. Suicide vector - (reply: 2)
1208. Extremely small bacterial colonies after transformation - (reply: 10)
1209. tranformation efficiency - (reply: 2)
1210. transfection time - (reply: 2)
1211. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1212. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1213. Problem with SmaI restriction digest - (reply: 6)
1214. blunt-end / 3ŽA overhang cloning - (reply: 1)
1215. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1216. overexpression of stable clones - (reply: 1)
1217. functionality of yeast promoter in E. coli - (reply: 1)
1218. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1219. Can't seem to do a simple cloning - (reply: 2)
1220. Histogram from Nanodrop - (reply: 1)
1221. cDNA library construction - I get only one colony (reply: 2)
1222. Cloning into pIBV5-His (not working) - (reply: 1)
1223. Addition of Restriction sites into PCR primers - (reply: 4)
1224. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1225. Streaky DNA digest - (reply: 3)
1226. TOPO TA cloning problem - (reply: 12)
1227. cloning problem - (reply: 2)
1228. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1229. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1230. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)