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631. Site-directed, PCR works but no colonies - (reply: 2)
632. Simoltanious Multi-restriction digestion - (reply: 3)
633. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
634. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
635. Restriction digestion problems - (reply: 3)
636. should I do blunt end my pcr product before ligation - (reply: 2)
637. Maxiprep of freeze/thawed pellet. - (reply: 1)
638. Digest with AarI and Pml I - (reply: 1)
639. Help with CGL gene cloning problems - (reply: 1)
640. screening for GPF - (reply: 10)
641. Creating a pGEM-T easy + insert sequence map - (reply: 1)
642. where to get vector overexpressing caveolin-1? - (reply: 2)
643. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
644. pINDUCER series - (reply: 1)
645. LZRS vector details - (reply: 1)
646. 12.4kb vector 6.7kb insert - (reply: 2)
647. Stable transfection - (reply: 2)
648. Using normal competent cells in electroporation method? - (reply: 1)
649. an effective way to do a yeast colony pcr - (reply: 2)
650. pGEM-T Easy vector contamination - (reply: 2)
651. recover old transformed DH5 - (reply: 1)
652. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
653. oligo(dt) 15 vs random primers - (reply: 3)
654. I need bynary vector pART27 - (reply: 1)
655. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
656. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
657. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
658. cloning into Pgem t easy - (reply: 1)
659. How to get pure protein of a gene - (reply: 3)
660. How to insert genes into chromosome of E. coli - (reply: 1)
632. Simoltanious Multi-restriction digestion - (reply: 3)
633. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
634. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
635. Restriction digestion problems - (reply: 3)
636. should I do blunt end my pcr product before ligation - (reply: 2)
637. Maxiprep of freeze/thawed pellet. - (reply: 1)
638. Digest with AarI and Pml I - (reply: 1)
639. Help with CGL gene cloning problems - (reply: 1)
640. screening for GPF - (reply: 10)
641. Creating a pGEM-T easy + insert sequence map - (reply: 1)
642. where to get vector overexpressing caveolin-1? - (reply: 2)
643. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
644. pINDUCER series - (reply: 1)
645. LZRS vector details - (reply: 1)
646. 12.4kb vector 6.7kb insert - (reply: 2)
647. Stable transfection - (reply: 2)
648. Using normal competent cells in electroporation method? - (reply: 1)
649. an effective way to do a yeast colony pcr - (reply: 2)
650. pGEM-T Easy vector contamination - (reply: 2)
651. recover old transformed DH5 - (reply: 1)
652. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
653. oligo(dt) 15 vs random primers - (reply: 3)
654. I need bynary vector pART27 - (reply: 1)
655. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
656. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
657. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
658. cloning into Pgem t easy - (reply: 1)
659. How to get pure protein of a gene - (reply: 3)
660. How to insert genes into chromosome of E. coli - (reply: 1)