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391. self ligation - (reply: 1)
392. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
393. Cloning large transgenes - (reply: 2)
394. In-fusion HD cloning - (reply: 5)
395. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
396. Few colonies frm sticky end cloning - (reply: 3)
397. transformation problem - (reply: 8)
398. Problems with Digestion? - (reply: 7)
399. plasmid digestion - problem with enzymes? - (reply: 4)
400. Problem with cloning - (reply: 7)
401. double transformation problem - (reply: 3)
402. Too many negative clones using Directional TOPO Cloning - (reply: 5)
403. competent cells - (reply: 1)
404. "Easiest" sticky end combination - (reply: 3)
405. Cloning advice - (reply: 3)
406. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
407. Cloning large fragments - (reply: 9)
408. Transformation colonies does not contain insert - (reply: 3)
409. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
410. Need urgent advise.. - (reply: 3)
411. Cloning Mitochondrial Targeting Sequence - (reply: 4)
412. problem in cloning - (reply: 2)
413. TA cloning: ligation problem or toxic construct? - (reply: 4)
414. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
415. Do I need to sequence after digestion? - (reply: 9)
416. quick change mutagenesis...... - (reply: 15)
417. Problem with double digestion: one enzyme is not working - (reply: 8)
418. Transient Transfection Controls (Gateway Technology) - (reply: 4)
419. Problems with crossover PCR - (reply: 2)
420. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
392. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
393. Cloning large transgenes - (reply: 2)
394. In-fusion HD cloning - (reply: 5)
395. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
396. Few colonies frm sticky end cloning - (reply: 3)
397. transformation problem - (reply: 8)
398. Problems with Digestion? - (reply: 7)
399. plasmid digestion - problem with enzymes? - (reply: 4)
400. Problem with cloning - (reply: 7)
401. double transformation problem - (reply: 3)
402. Too many negative clones using Directional TOPO Cloning - (reply: 5)
403. competent cells - (reply: 1)
404. "Easiest" sticky end combination - (reply: 3)
405. Cloning advice - (reply: 3)
406. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
407. Cloning large fragments - (reply: 9)
408. Transformation colonies does not contain insert - (reply: 3)
409. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
410. Need urgent advise.. - (reply: 3)
411. Cloning Mitochondrial Targeting Sequence - (reply: 4)
412. problem in cloning - (reply: 2)
413. TA cloning: ligation problem or toxic construct? - (reply: 4)
414. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
415. Do I need to sequence after digestion? - (reply: 9)
416. quick change mutagenesis...... - (reply: 15)
417. Problem with double digestion: one enzyme is not working - (reply: 8)
418. Transient Transfection Controls (Gateway Technology) - (reply: 4)
419. Problems with crossover PCR - (reply: 2)
420. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)