721. Ligation of insert to itself problem - (reply: 3)
722. double digest problem - (reply: 3)
723. Difficulty in expressing fusion protein - (reply: 1)
724. Plasmid recovery problem - (reply: 3)
725. Restriction Digest Problem - (reply: 5)
726. Recombination protocol needed - (reply: 2)
727. Cloning problem using pET32a vector - (reply: 3)
728. Fixing SNPs after cloning - (reply: 5)
729. Electroporated plasmid loses the insert - (reply: 8)
730. Recombination due to overselective plate? - (reply: 11)
731. Help with ligation - (reply: 2)
732. emty vector cloning - (reply: 3)
733. RE site on complimentary strand of plasmid? - (reply: 1)
734. LENTIVIRUS transfection efficiency - (reply: 1)
735. Double digest after cloning doesn't give gene - (reply: 6)
736. Blunt end PCR product ligation - (reply: 1)
737. Screening for clones - (reply: 2)
738. Plasmid isolation Qiagen - (reply: 2)
739. Restriction Site Destruction - (reply: 1)
740. The copy number of pPICZ alpha A - (reply: 6)
741. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
742. need help with subcloning !! - (reply: 2)
743. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
744. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
745. cdna library. - (reply: 2)
746. Help in RE digestion of vector - (reply: 5)
747. bacterial transformation problem - (reply: 2)
748. Transformation - (reply: 3)
749. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
750. What does vortexing do in transformation? - (reply: 1)

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