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121. transformation - (reply: 2)
122. problem in sequencing of gene - (reply: 3)
123. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
124. Plasmid Methylation - (reply: 3)
125. Problem with BamHI Digestion - (reply: 7)
126. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
127. How to find promoter sequence ... - (reply: 3)
128. Sticky and blunt end ligation in single reaction? - (reply: 2)
129. Restriction double digest - (reply: 1)
130. Choice of AMP 100 versus AMP 50 - (reply: 1)
131. Why are there two origin of replications in plasmids - (reply: 3)
132. How can we expand molecular cloning further? - (reply: 3)
133. Subtle change in insert size after cloning - (reply: 6)
134. lactic acid bacteria - cloning - (reply: 2)
135. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
136. All I get is supercoiled DNA... - (reply: 5)
137. Help Quesion - (reply: 1)
138. No colonies after self Circularization ligation - (reply: 2)
139. Clean ligation controls but no insert - (reply: 14)
140. ratios insert/vector - (reply: 3)
141. Can't isolate plasmid from cloned E.coli - (reply: 4)
142. Genomic DNA quantification using the NanoDrop - (reply: 3)
143. Competent cells with Hanahan's method - (reply: 2)
144. cloning issues - (reply: 3)
145. Is important the direction of IRES-GFP in cloning? - (reply: 1)
146. What are the error-rates of the TA cloning Kits? - (reply: 8)
147. Can mutations be created during cloning? - (reply: 7)
148. confusing with where to put primers and which ordination - (reply: 4)
149. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
150. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
122. problem in sequencing of gene - (reply: 3)
123. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
124. Plasmid Methylation - (reply: 3)
125. Problem with BamHI Digestion - (reply: 7)
126. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
127. How to find promoter sequence ... - (reply: 3)
128. Sticky and blunt end ligation in single reaction? - (reply: 2)
129. Restriction double digest - (reply: 1)
130. Choice of AMP 100 versus AMP 50 - (reply: 1)
131. Why are there two origin of replications in plasmids - (reply: 3)
132. How can we expand molecular cloning further? - (reply: 3)
133. Subtle change in insert size after cloning - (reply: 6)
134. lactic acid bacteria - cloning - (reply: 2)
135. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
136. All I get is supercoiled DNA... - (reply: 5)
137. Help Quesion - (reply: 1)
138. No colonies after self Circularization ligation - (reply: 2)
139. Clean ligation controls but no insert - (reply: 14)
140. ratios insert/vector - (reply: 3)
141. Can't isolate plasmid from cloned E.coli - (reply: 4)
142. Genomic DNA quantification using the NanoDrop - (reply: 3)
143. Competent cells with Hanahan's method - (reply: 2)
144. cloning issues - (reply: 3)
145. Is important the direction of IRES-GFP in cloning? - (reply: 1)
146. What are the error-rates of the TA cloning Kits? - (reply: 8)
147. Can mutations be created during cloning? - (reply: 7)
148. confusing with where to put primers and which ordination - (reply: 4)
149. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
150. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)