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901. restriction digestion against colony PCR - (reply: 1)
902. BAC for transgenic mice - (reply: 6)
903. Tag proteins for CO-IP and confocal - (reply: 1)
904. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
905. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
906. how long for a double digestion - (reply: 2)
907. Problems with Site-Directed Mutagenesis - (reply: 2)
908. blunt and sticky end clonning temperature - (reply: 2)
909. Most common mistakes - What mistakes are made during cloning? (reply: 7)
910. Luciferase Assay Stim. Concentration? - (reply: 1)
911. Cloning direction / orientation? - (reply: 2)
912. Question about plasmid midiprep - (reply: 1)
913. Does a polyA sequence need to be put in-frame? - (reply: 1)
914. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
915. Problem on growing up bugs - (reply: 8)
916. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
917. pSIM in BL21(DE3) - (reply: 2)
918. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
919. Weird problem in invitromutagenesis - (reply: 3)
920. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
921. RE digestion - (reply: 4)
922. HELP!!! - (reply: 3)
923. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
924. Cloning of ITS region - (reply: 6)
925. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
926. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
927. SmaI blunt end ligation problem... - (reply: 3)
928. AAV construct? - (reply: 1)
929. No bands on agarose gel after restriction digestion. - (reply: 10)
930. inverse pcr - (reply: 2)
902. BAC for transgenic mice - (reply: 6)
903. Tag proteins for CO-IP and confocal - (reply: 1)
904. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
905. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
906. how long for a double digestion - (reply: 2)
907. Problems with Site-Directed Mutagenesis - (reply: 2)
908. blunt and sticky end clonning temperature - (reply: 2)
909. Most common mistakes - What mistakes are made during cloning? (reply: 7)
910. Luciferase Assay Stim. Concentration? - (reply: 1)
911. Cloning direction / orientation? - (reply: 2)
912. Question about plasmid midiprep - (reply: 1)
913. Does a polyA sequence need to be put in-frame? - (reply: 1)
914. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
915. Problem on growing up bugs - (reply: 8)
916. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
917. pSIM in BL21(DE3) - (reply: 2)
918. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
919. Weird problem in invitromutagenesis - (reply: 3)
920. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
921. RE digestion - (reply: 4)
922. HELP!!! - (reply: 3)
923. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
924. Cloning of ITS region - (reply: 6)
925. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
926. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
927. SmaI blunt end ligation problem... - (reply: 3)
928. AAV construct? - (reply: 1)
929. No bands on agarose gel after restriction digestion. - (reply: 10)
930. inverse pcr - (reply: 2)