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1. pKOV ligation - (reply: 1)
2. Antibiotic not working in LB agar, fine in broth - (reply: 3)
3. TA cloning for sars cov 2 nucleocapsid protein. - (reply: 4)
4. Generating a cell line using sleeping beauty transposase and a gene that contain - (reply: 1)
5. HELP: Validation of a polyclonal knock out (KO) cell line when you dont have a g - (reply: 6)
6. Trouble transforming pET28a in E. coli BL21(DE3) - (reply: 5)
7. Choosing a common exon in GOI to design your guides - (reply: 1)
8. Designing Crispr guides and cloning into appropriate vectors - (reply: 1)
9. plasmid purification - (reply: 4)
10. Cloning protein of interest into lentiviral vector - help - I have no experience - (reply: 20)
11. Insert in frame - (reply: 1)
12. PCRed-vector approach to tag switching - (reply: 1)
13. High copy vs low copy plasmids: which one causes less toxicity? - (reply: 2)
14. Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
15. low copy number plasmid purification kit - (reply: 4)
16. Neomycin/Kanamycin Resistant gene - (reply: 2)
17. Plasmid analysis in limited conditions - (reply: 6)
18. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
19. Troubleshooting with restriction enzymes during cloning - (reply: 5)
20. Transformation - (reply: 3)
21. Plasmid distributors for Luciferase Assay? - (reply: 2)
22. Ligate a very small insert into a vector - (reply: 3)
23. Restriction enzyme - (reply: 1)
24. Plasmid dna size - (reply: 3)
25. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
26. Express two different membrane proteins on the same promoter - (reply: 1)
27. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
28. [Overexpression] Without upstream transcription terminator - (reply: 4)
29. How large need homology arms to be? - (reply: 1)
30. Ampicillin LB agar reheating and reusing - (reply: 2)
2. Antibiotic not working in LB agar, fine in broth - (reply: 3)
3. TA cloning for sars cov 2 nucleocapsid protein. - (reply: 4)
4. Generating a cell line using sleeping beauty transposase and a gene that contain - (reply: 1)
5. HELP: Validation of a polyclonal knock out (KO) cell line when you dont have a g - (reply: 6)
6. Trouble transforming pET28a in E. coli BL21(DE3) - (reply: 5)
7. Choosing a common exon in GOI to design your guides - (reply: 1)
8. Designing Crispr guides and cloning into appropriate vectors - (reply: 1)
9. plasmid purification - (reply: 4)
10. Cloning protein of interest into lentiviral vector - help - I have no experience - (reply: 20)
11. Insert in frame - (reply: 1)
12. PCRed-vector approach to tag switching - (reply: 1)
13. High copy vs low copy plasmids: which one causes less toxicity? - (reply: 2)
14. Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
15. low copy number plasmid purification kit - (reply: 4)
16. Neomycin/Kanamycin Resistant gene - (reply: 2)
17. Plasmid analysis in limited conditions - (reply: 6)
18. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
19. Troubleshooting with restriction enzymes during cloning - (reply: 5)
20. Transformation - (reply: 3)
21. Plasmid distributors for Luciferase Assay? - (reply: 2)
22. Ligate a very small insert into a vector - (reply: 3)
23. Restriction enzyme - (reply: 1)
24. Plasmid dna size - (reply: 3)
25. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
26. Express two different membrane proteins on the same promoter - (reply: 1)
27. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
28. [Overexpression] Without upstream transcription terminator - (reply: 4)
29. How large need homology arms to be? - (reply: 1)
30. Ampicillin LB agar reheating and reusing - (reply: 2)