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751. failure in miniprep - (reply: 1)
752. Host cells for transformation - (reply: 3)
753. making recombinant protein - (reply: 2)
754. Confirming insert???? - (reply: 1)
755. how to make a recombinant protein with signal peptide - (reply: 1)
756. How to add restriction enzyme site into the DNA fragment - (reply: 3)
757. Issues with ligation - (reply: 2)
758. Ligation of double insert into pET31 failing - (reply: 1)
759. Homemade MiniPreps for Plasmid Purification - (reply: 1)
760. Agar has dried out during cultivation - (reply: 1)
761. Gateway cloning vector compatibility - (reply: 2)
762. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
763. Cloning of IL8-promoter - (reply: 3)
764. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
765. Template DNA (plasmid) concentration for PCR - (reply: 4)
766. Ligation of 16kb PCR product - (reply: 5)
767. stable transfection - (reply: 1)
768. How to choose the right plasmids for certain types of cell? - (reply: 1)
769. Ligation needed after SDM with PfuX? - (reply: 3)
770. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
771. Cloning promoter (overlap with another gene!) - (reply: 3)
772. bacterial transfeomation - (reply: 2)
773. Loss of insert? - (reply: 1)
774. Sequencing trouble - (reply: 7)
775. Platinum-A cell trasnfection toruble - (reply: 1)
776. Cloning a promoter - (reply: 2)
777. pcDNA 3.1 His Expression - (reply: 2)
778. Cloning two fragments into a vector - (reply: 3)
779. confirmation of insert in to vector - (reply: 3)
780. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
752. Host cells for transformation - (reply: 3)
753. making recombinant protein - (reply: 2)
754. Confirming insert???? - (reply: 1)
755. how to make a recombinant protein with signal peptide - (reply: 1)
756. How to add restriction enzyme site into the DNA fragment - (reply: 3)
757. Issues with ligation - (reply: 2)
758. Ligation of double insert into pET31 failing - (reply: 1)
759. Homemade MiniPreps for Plasmid Purification - (reply: 1)
760. Agar has dried out during cultivation - (reply: 1)
761. Gateway cloning vector compatibility - (reply: 2)
762. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
763. Cloning of IL8-promoter - (reply: 3)
764. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
765. Template DNA (plasmid) concentration for PCR - (reply: 4)
766. Ligation of 16kb PCR product - (reply: 5)
767. stable transfection - (reply: 1)
768. How to choose the right plasmids for certain types of cell? - (reply: 1)
769. Ligation needed after SDM with PfuX? - (reply: 3)
770. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
771. Cloning promoter (overlap with another gene!) - (reply: 3)
772. bacterial transfeomation - (reply: 2)
773. Loss of insert? - (reply: 1)
774. Sequencing trouble - (reply: 7)
775. Platinum-A cell trasnfection toruble - (reply: 1)
776. Cloning a promoter - (reply: 2)
777. pcDNA 3.1 His Expression - (reply: 2)
778. Cloning two fragments into a vector - (reply: 3)
779. confirmation of insert in to vector - (reply: 3)
780. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)