151. RBS - (reply: 1)
152. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
153. Gel for my ligation - (reply: 1)
154. Amplifying from pcr products - (reply: 7)
155. Design the primers - (reply: 10)
156. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
157. How two fragments joint together when doing fusion PCR? - (reply: 4)
158. Working backwards from a construct? - (reply: 1)
159. disappearing bases - (reply: 4)
160. Mutagenesis PCR - (reply: 9)
161. Digest two sites located next to each other - (reply: 5)
162. cDNA Library Construction by using kits and Sequencing - (reply: 1)
163. Looking for software that permutes codons to find new restriction sites - (reply: 4)
164. Transforming dna from agarose gel - (reply: 3)
165. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
166. ccdb toxin - (reply: 15)
167. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
168. annealing oligos - (reply: 3)
169. Does a restriction enzyme cut at a related site? - (reply: 5)
170. primers for cloning - (reply: 1)
171. Vector-insert ratio - (reply: 6)
172. Cloning contamination - (reply: 3)
173. plasmid construction - (reply: 2)
174. primer design with restriction enzyme - (reply: 1)
175. vector propagation in E coli - (reply: 1)
176. Restriction to remove background - (reply: 4)
177. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
178. Restriction and ligation time interval - (reply: 1)
179. Problem shrna cloning, bad sequencing results - (reply: 1)
180. Lentivirus copy number - (reply: 7)

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