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691. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
692. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
693. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
694. Plasmid digestion after transformation - (reply: 4)
695. Blunt end ligation insert:vector ratio - (reply: 1)
696. PCRed on restriction sites -- how do I purify it? - (reply: 8)
697. Puzzle about plamid and its digest, plz help - (reply: 8)
698. Smear above plasmid dna - (reply: 3)
699. restriction digestion buffers contaminated with dnase - (reply: 3)
700. Bacterial Induction - (reply: 1)
701. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
702. smear restriction digest - (reply: 1)
703. How to set the Sonicator to 20%amplitude? - (reply: 3)
704. No DNA after midiprep - (reply: 2)
705. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
706. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
707. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
708. Impact of primer sequence on TA cloning - (reply: 3)
709. Maximum length of cDNA from RT reaction - (reply: 6)
710. overlapping PCR protocol - (reply: 12)
711. cloning an AT rich gene - (reply: 3)
712. Cloning two genes in the same plasmid - (reply: 4)
713. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
714. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
715. Problem: Cloning DNA shuffled band - (reply: 2)
716. shRNA to primary keratinocytes - (reply: 1)
717. Enzymetic digestion with ApaI and HincII - (reply: 2)
718. Ligation gel picture interpretation - (reply: 4)
719. strange subcloning problem - (reply: 9)
720. Cloned sequence is not predicted size by western blot - (reply: 1)
692. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
693. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
694. Plasmid digestion after transformation - (reply: 4)
695. Blunt end ligation insert:vector ratio - (reply: 1)
696. PCRed on restriction sites -- how do I purify it? - (reply: 8)
697. Puzzle about plamid and its digest, plz help - (reply: 8)
698. Smear above plasmid dna - (reply: 3)
699. restriction digestion buffers contaminated with dnase - (reply: 3)
700. Bacterial Induction - (reply: 1)
701. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
702. smear restriction digest - (reply: 1)
703. How to set the Sonicator to 20%amplitude? - (reply: 3)
704. No DNA after midiprep - (reply: 2)
705. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
706. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
707. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
708. Impact of primer sequence on TA cloning - (reply: 3)
709. Maximum length of cDNA from RT reaction - (reply: 6)
710. overlapping PCR protocol - (reply: 12)
711. cloning an AT rich gene - (reply: 3)
712. Cloning two genes in the same plasmid - (reply: 4)
713. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
714. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
715. Problem: Cloning DNA shuffled band - (reply: 2)
716. shRNA to primary keratinocytes - (reply: 1)
717. Enzymetic digestion with ApaI and HincII - (reply: 2)
718. Ligation gel picture interpretation - (reply: 4)
719. strange subcloning problem - (reply: 9)
720. Cloned sequence is not predicted size by western blot - (reply: 1)