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241. Lac Promoter activity in P. aeruginosa - (reply: 1)
242. Resctriction analysis - (reply: 5)
243. Please comment on my Cloning Plan - (reply: 1)
244. Gibson/SLIC question - (reply: 1)
245. How to determine where, in the genome, my transgene inserted - (reply: 4)
246. no colonies, ever! - (reply: 5)
247. fluorescent tag on membrane protein - (reply: 2)
248. How can addgene sell plasmids with patented technology - (reply: 2)
249. no insert issue - (reply: 9)
250. Considerations on the design of a transgene - (reply: 1)
251. How to obtain Vectors used in articles - (reply: 5)
252. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
253. How efficient is the 2A sequence - (reply: 2)
254. Transformation keeps failing - (reply: 2)
255. High background issue - (reply: 5)
256. Pcr primers - (reply: 7)
257. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
258. Gateway vectors - (reply: 3)
259. question on gateway - (reply: 1)
260. Cloning problem still not solved - (reply: 2)
261. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
262. Insert 30nt at C-terminal - (reply: 1)
263. How to store a ligation reaction over the weekend? - (reply: 3)
264. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
265. How homologous do homology arms need to be? - (reply: 3)
266. cloning pEGFP-N1 - (reply: 1)
267. Loss of DNA after restriction digestion cleanup - (reply: 2)
268. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
269. separating mixture of plamids - (reply: 7)
270. Kozak sequence and mammalian expression vector - (reply: 3)
242. Resctriction analysis - (reply: 5)
243. Please comment on my Cloning Plan - (reply: 1)
244. Gibson/SLIC question - (reply: 1)
245. How to determine where, in the genome, my transgene inserted - (reply: 4)
246. no colonies, ever! - (reply: 5)
247. fluorescent tag on membrane protein - (reply: 2)
248. How can addgene sell plasmids with patented technology - (reply: 2)
249. no insert issue - (reply: 9)
250. Considerations on the design of a transgene - (reply: 1)
251. How to obtain Vectors used in articles - (reply: 5)
252. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
253. How efficient is the 2A sequence - (reply: 2)
254. Transformation keeps failing - (reply: 2)
255. High background issue - (reply: 5)
256. Pcr primers - (reply: 7)
257. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
258. Gateway vectors - (reply: 3)
259. question on gateway - (reply: 1)
260. Cloning problem still not solved - (reply: 2)
261. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
262. Insert 30nt at C-terminal - (reply: 1)
263. How to store a ligation reaction over the weekend? - (reply: 3)
264. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
265. How homologous do homology arms need to be? - (reply: 3)
266. cloning pEGFP-N1 - (reply: 1)
267. Loss of DNA after restriction digestion cleanup - (reply: 2)
268. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
269. separating mixture of plamids - (reply: 7)
270. Kozak sequence and mammalian expression vector - (reply: 3)