|
1621. ligation problem - (reply: 3)
1622. ligation question - three-point ligation (reply: 9)
1623. Problems cloning Hairpin vector - (reply: 3)
1624. request pIRES2-EGFP - (reply: 2)
1625. different digestion pattern for same clone - (reply: 4)
1626. Problem with DNA purification from agarose gel - (reply: 3)
1627. cloning trouble - (reply: 15)
1628. No band in the gel after ligation - (reply: 6)
1629. Troubleshooting Digest, ligation, transformation - (reply: 7)
1630. Ideal incubation time - (reply: 3)
1631. Competent DH5 alpha - (reply: 4)
1632. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
1633. Quick ? about Enzyme Buffers - (reply: 3)
1634. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1635. Inserts too small after transformation - transformation (reply: 3)
1636. glycerol stocks - storage (reply: 2)
1637. growth of colonies after ligation - (reply: 2)
1638. cDNA cloning problem - (reply: 1)
1639. Any good way to remove DNase? - (reply: 5)
1640. DH5a comp cells - (reply: 5)
1641. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
1642. Monocot promoters - (reply: 1)
1643. ligation problem - (reply: 11)
1644. How Gateway cloning system works? - (reply: 1)
1645. Chemically competent to electrocompetent DH5alpha - (reply: 1)
1646. Tetracycline concnetration in selection - (reply: 1)
1647. pCS2 - (reply: 2)
1648. What does this look like to you? - (reply: 23)
1649. ds-oligo cloning/ligation issues - (reply: 18)
1650. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
1622. ligation question - three-point ligation (reply: 9)
1623. Problems cloning Hairpin vector - (reply: 3)
1624. request pIRES2-EGFP - (reply: 2)
1625. different digestion pattern for same clone - (reply: 4)
1626. Problem with DNA purification from agarose gel - (reply: 3)
1627. cloning trouble - (reply: 15)
1628. No band in the gel after ligation - (reply: 6)
1629. Troubleshooting Digest, ligation, transformation - (reply: 7)
1630. Ideal incubation time - (reply: 3)
1631. Competent DH5 alpha - (reply: 4)
1632. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
1633. Quick ? about Enzyme Buffers - (reply: 3)
1634. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1635. Inserts too small after transformation - transformation (reply: 3)
1636. glycerol stocks - storage (reply: 2)
1637. growth of colonies after ligation - (reply: 2)
1638. cDNA cloning problem - (reply: 1)
1639. Any good way to remove DNase? - (reply: 5)
1640. DH5a comp cells - (reply: 5)
1641. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
1642. Monocot promoters - (reply: 1)
1643. ligation problem - (reply: 11)
1644. How Gateway cloning system works? - (reply: 1)
1645. Chemically competent to electrocompetent DH5alpha - (reply: 1)
1646. Tetracycline concnetration in selection - (reply: 1)
1647. pCS2 - (reply: 2)
1648. What does this look like to you? - (reply: 23)
1649. ds-oligo cloning/ligation issues - (reply: 18)
1650. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)