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661. Gene synthesis - (reply: 2)
662. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
663. Pls help me with this plasmid!!! - (reply: 1)
664. Problems with Restriction Digest Results - (reply: 7)
665. Methods of plasmid transformation? only heat shock? - (reply: 1)
666. Digestion - (reply: 2)
667. Digestion of Genomic DNA - (reply: 1)
668. Isolation of a Gene of Interest for Subcloning - (reply: 8)
669. E.coli grow on neg. control - (reply: 1)
670. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
671. Need help troubleshooting digest. - (reply: 1)
672. Competent cells question: JM101 vs DH5alpha - (reply: 2)
673. How to avoid plasmid instability - (reply: 4)
674. Question Help - (reply: 1)
675. Shear-induced damage of plasmid by vortexing - (reply: 1)
676. Problems with blunt end ligation - (reply: 3)
677. Problem with Transforming E. coli DH10Bac - (reply: 8)
678. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
679. Heat inactivate ligase - (reply: 5)
680. DMSO stock of overnight bacterial cultures - (reply: 1)
681. Recommended competent cell for transformation of large plasmids? - (reply: 4)
682. Prolem in screening of hygromycin concentration - (reply: 1)
683. Primers for Introduction of new restriction sites to a vector - (reply: 3)
684. problem in cloning involving partial digestion - (reply: 1)
685. Why perform restriction enzyme digestion again? - (reply: 1)
686. Cotransient Transfections DNA Requirements - (reply: 3)
687. which software to draw the scheme of cloning strategy - (reply: 5)
688. Help with restriction analysis - (reply: 1)
689. Sequencing of PCR product - (reply: 4)
690. KanR sequence - (reply: 2)
662. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
663. Pls help me with this plasmid!!! - (reply: 1)
664. Problems with Restriction Digest Results - (reply: 7)
665. Methods of plasmid transformation? only heat shock? - (reply: 1)
666. Digestion - (reply: 2)
667. Digestion of Genomic DNA - (reply: 1)
668. Isolation of a Gene of Interest for Subcloning - (reply: 8)
669. E.coli grow on neg. control - (reply: 1)
670. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
671. Need help troubleshooting digest. - (reply: 1)
672. Competent cells question: JM101 vs DH5alpha - (reply: 2)
673. How to avoid plasmid instability - (reply: 4)
674. Question Help - (reply: 1)
675. Shear-induced damage of plasmid by vortexing - (reply: 1)
676. Problems with blunt end ligation - (reply: 3)
677. Problem with Transforming E. coli DH10Bac - (reply: 8)
678. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
679. Heat inactivate ligase - (reply: 5)
680. DMSO stock of overnight bacterial cultures - (reply: 1)
681. Recommended competent cell for transformation of large plasmids? - (reply: 4)
682. Prolem in screening of hygromycin concentration - (reply: 1)
683. Primers for Introduction of new restriction sites to a vector - (reply: 3)
684. problem in cloning involving partial digestion - (reply: 1)
685. Why perform restriction enzyme digestion again? - (reply: 1)
686. Cotransient Transfections DNA Requirements - (reply: 3)
687. which software to draw the scheme of cloning strategy - (reply: 5)
688. Help with restriction analysis - (reply: 1)
689. Sequencing of PCR product - (reply: 4)
690. KanR sequence - (reply: 2)