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Molecular-Cloning
1591.
Ligation issues -
(reply: 4)
1592.
messed up restriction sites -
(reply: 3)
1593.
annoying PCR cloning problem (season 2) -
(reply: 5)
1594.
Cloning Issues with Rest Digest/Ligation/Transformation -
(reply: 2)
1595.
size limit of plasmid origins -
(reply: 2)
1596.
Problems with Stratagene Site Directed Mutagenesis -
(reply: 36)
1597.
confusion about 5'UTR - confusion about 5'UTR
(reply: 1)
1598.
qPCR - urgent help
(reply: 2)
1599.
GFP -
(reply: 2)
1600.
Creating empty vector by self-ligation -
(reply: 31)
1601.
ligation probs -
(reply: 2)
1602.
Insert phosphorylation upon Klenow? -
(reply: 5)
1603.
confusion with different forms of plasmid -
(reply: 3)
1604.
an annoying cloning -
(reply: 9)
1605.
dpn1 digest -
(reply: 5)
1606.
Controls for TOPO cloning? -
(reply: 1)
1607.
primers fpr sequencing -
(reply: 4)
1608.
help with ligation/transformation -
(reply: 1)
1609.
ligation problem with SalI degested vector and insert -
(reply: 6)
1610.
IRES1 x IRES2? -
(reply: 5)
1611.
Restriction site - efficiency of restriction
(reply: 4)
1612.
lentivirus vector problem--help help -
(reply: 6)
1613.
A problem with deleting URA3 from S. cerevisiae -
(reply: 2)
1614.
Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation
(reply: 3)
1615.
ligation - didn't got transformed colonies
(reply: 1)
1616.
What's this in my culture??? - Debris in liquid culture
(reply: 3)
1617.
assembling problem in genes - assembling problem in genes
(reply: 7)
1618.
tryptone soy broth -
(reply: 3)
1619.
no colonies - Cloning using pENTR vector, no colonies
(reply: 2)
1620.
Please help Cloning issues with smaI and pUNK1 vector -
(reply: 2)
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