|
1561. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1562. How to transfect two plasmids in a cell - (reply: 9)
1563. Double Digest using enzymes from diff companies - (reply: 4)
1564. Ligation problem (no colonies, but on negative control) - (reply: 3)
1565. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1566. SDM - (reply: 3)
1567. inframe problem - (reply: 1)
1568. Touchdown PCR - (reply: 1)
1569. Adding A tails? - (reply: 3)
1570. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1571. Determine annealing temperature of primers - (reply: 14)
1572. Bacteria pellet has almost-black region - (reply: 10)
1573. no ligation !!!!! - (reply: 6)
1574. How to clean up restriction enzyme digestion - (reply: 8)
1575. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1576. GPCR - (reply: 1)
1577. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1578. finding the suitable RE - first timer (reply: 6)
1579. TOPO TA Problems - TOPO TA Problems (reply: 8)
1580. about antibiotic addition - (reply: 4)
1581. about chloramphenicol and L.lactis - (reply: 1)
1582. ligation - (reply: 5)
1583. Losing insert from pGEM? - (reply: 2)
1584. How to concentrate lenti or retroviral particules - (reply: 3)
1585. wierd clot in LB culture - (reply: 5)
1586. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1587. Not getting enough colony - (reply: 4)
1588. Ligation problem - (reply: 9)
1589. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
1590. about flanking sequnce - (reply: 3)
1562. How to transfect two plasmids in a cell - (reply: 9)
1563. Double Digest using enzymes from diff companies - (reply: 4)
1564. Ligation problem (no colonies, but on negative control) - (reply: 3)
1565. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1566. SDM - (reply: 3)
1567. inframe problem - (reply: 1)
1568. Touchdown PCR - (reply: 1)
1569. Adding A tails? - (reply: 3)
1570. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1571. Determine annealing temperature of primers - (reply: 14)
1572. Bacteria pellet has almost-black region - (reply: 10)
1573. no ligation !!!!! - (reply: 6)
1574. How to clean up restriction enzyme digestion - (reply: 8)
1575. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1576. GPCR - (reply: 1)
1577. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1578. finding the suitable RE - first timer (reply: 6)
1579. TOPO TA Problems - TOPO TA Problems (reply: 8)
1580. about antibiotic addition - (reply: 4)
1581. about chloramphenicol and L.lactis - (reply: 1)
1582. ligation - (reply: 5)
1583. Losing insert from pGEM? - (reply: 2)
1584. How to concentrate lenti or retroviral particules - (reply: 3)
1585. wierd clot in LB culture - (reply: 5)
1586. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1587. Not getting enough colony - (reply: 4)
1588. Ligation problem - (reply: 9)
1589. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
1590. about flanking sequnce - (reply: 3)