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421. Two genes, same MCS - (reply: 1)
422. Including ATG in cloning? - (reply: 1)
423. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
424. Low copy vector for library generation - (reply: 2)
425. Can't find a recombinant after ligating - (reply: 4)
426. problems with plasmid miniprep - (reply: 8)
427. gene cloning - (reply: 1)
428. Is ligation really necessary? - (reply: 6)
429. problem with ligation of linker/adaptor - (reply: 2)
430. GFP -weak signal -why? - (reply: 1)
431. Ligation troubleshooting! Please help! - (reply: 5)
432. Truncated gene Cloning - (reply: 1)
433. Screening for ligation and transformation result - (reply: 2)
434. Problem with 3 way ligation - (reply: 2)
435. Query regarding primers for quick change mutagenesis - (reply: 3)
436. What is NEB's CutSmart Buffer? - (reply: 5)
437. Enzyme cutting: low efficiency - (reply: 5)
438. Issue with Xba1 and Dam methylation - (reply: 7)
439. Restriction site rearrangement - (reply: 1)
440. Mutation in cloning - (reply: 6)
441. Must the terminator be in frame? - (reply: 1)
442. sequencing problems - (reply: 2)
443. Understand the basics of molecular cloning - (reply: 6)
444. Maintainence of transformed cells - (reply: 5)
445. Restriction digest band shift? - (reply: 1)
446. problem in cloning PCR primer design with restriction site - (reply: 4)
447. quick change mutagenesis...... - (reply: 1)
448. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
449. Help for pGEM-T easy vector ligation problem - (reply: 2)
450. Co-transformation - (reply: 2)
422. Including ATG in cloning? - (reply: 1)
423. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
424. Low copy vector for library generation - (reply: 2)
425. Can't find a recombinant after ligating - (reply: 4)
426. problems with plasmid miniprep - (reply: 8)
427. gene cloning - (reply: 1)
428. Is ligation really necessary? - (reply: 6)
429. problem with ligation of linker/adaptor - (reply: 2)
430. GFP -weak signal -why? - (reply: 1)
431. Ligation troubleshooting! Please help! - (reply: 5)
432. Truncated gene Cloning - (reply: 1)
433. Screening for ligation and transformation result - (reply: 2)
434. Problem with 3 way ligation - (reply: 2)
435. Query regarding primers for quick change mutagenesis - (reply: 3)
436. What is NEB's CutSmart Buffer? - (reply: 5)
437. Enzyme cutting: low efficiency - (reply: 5)
438. Issue with Xba1 and Dam methylation - (reply: 7)
439. Restriction site rearrangement - (reply: 1)
440. Mutation in cloning - (reply: 6)
441. Must the terminator be in frame? - (reply: 1)
442. sequencing problems - (reply: 2)
443. Understand the basics of molecular cloning - (reply: 6)
444. Maintainence of transformed cells - (reply: 5)
445. Restriction digest band shift? - (reply: 1)
446. problem in cloning PCR primer design with restriction site - (reply: 4)
447. quick change mutagenesis...... - (reply: 1)
448. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
449. Help for pGEM-T easy vector ligation problem - (reply: 2)
450. Co-transformation - (reply: 2)