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Molecular-Cloning
211.
sequencing getting wrong 1 nucleotide :((( -
(reply: 4)
212.
How to clone a gene whose genome has not been sequenced yet? -
(reply: 4)
213.
restriction enzymes/sites to avoid -
(reply: 4)
214.
Using pBABE plasmids for regular transfection -
(reply: 1)
215.
Ubiquitin promoter cloning -
(reply: 1)
216.
Cloning two genes in the same plasmid with one promotor -
(reply: 4)
217.
Sending a TOPO-construct for sequencing? -
(reply: 1)
218.
Is this counter-productive cloning or not? -
(reply: 8)
219.
Colony screening -
(reply: 1)
220.
Failed cloning for 1month - TOPO -
(reply: 7)
221.
A modified method for blunt end DNA cloning!!!!!!! -
(reply: 2)
222.
Which method for DNA purification b4 cloning -
(reply: 4)
223.
Purification for TOPO -
(reply: 1)
224.
Designing cloning primes (restriction site and kozak sequence) -
(reply: 1)
225.
Failed TOPO cloning - what happened? -
(reply: 9)
226.
TOPO cloning after Phusion-PCR -
(reply: 1)
227.
Gateway cloning-modifications to "entry" plasmid -
(reply: 4)
228.
How to improve yield in DNA-purification from agars electrophoresis? -
(reply: 3)
229.
Deleting 7Mbp chromosomal region -
(reply: 3)
230.
Electrophoresis samples and volumes -
(reply: 1)
231.
Gene cloning with unknown sequence -
(reply: 6)
232.
Checking for efficieny competent cells -
(reply: 1)
233.
Troubles with Fusion PCR -
(reply: 1)
234.
Does this look alright? -
(reply: 3)
235.
Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? -
(reply: 1)
236.
Cloning pDsRed-Monomer-N1 -
(reply: 1)
237.
forgot to heat shock -
(reply: 6)
238.
Ligation left for weekend at room temp -
(reply: 4)
239.
E.coli culture in -4c for 2 hours means it will affect or not? what happened in -
(reply: 3)
240.
Problem in plasmid digestion after transformation -
(reply: 4)
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