|
811. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
812. Three-way ligation in two steps? - (reply: 3)
813. How does circular, uncut pGEM-T migrate? - (reply: 2)
814. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
815. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
816. Cloning App Released from Life Technologies - (reply: 2)
817. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
818. Dissolve IPTG in EtOH - (reply: 4)
819. problem with transient transfection - (reply: 6)
820. Recombinant construct looks smaller than empty vector - (reply: 3)
821. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
822. hygromycin concentration for e-coli selection - (reply: 1)
823. orientation of promoter into viral construct - (reply: 1)
824. problems with PCR confirmation of insert . HELP ! - (reply: 1)
825. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
826. Loss of promoter during ligation - (reply: 2)
827. Enzyme restriction - (reply: 8)
828. Colony PCR Problem!!!! - (reply: 5)
829. Removing unwanted sequence from pUC19 - (reply: 4)
830. transformation fails, bacteria grows in control plate - (reply: 6)
831. competentce cell, cloning - (reply: 2)
832. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
833. co-transformation - (reply: 3)
834. pcr clean-up fail? - (reply: 1)
835. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
836. sequential cloning of multiple PCR products - how to do? (reply: 3)
837. Cloning large gene - (reply: 3)
838. Glycosilated peptide's cDNA cloning - (reply: 3)
839. pWM91 cloning problem - (reply: 4)
840. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
812. Three-way ligation in two steps? - (reply: 3)
813. How does circular, uncut pGEM-T migrate? - (reply: 2)
814. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
815. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
816. Cloning App Released from Life Technologies - (reply: 2)
817. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
818. Dissolve IPTG in EtOH - (reply: 4)
819. problem with transient transfection - (reply: 6)
820. Recombinant construct looks smaller than empty vector - (reply: 3)
821. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
822. hygromycin concentration for e-coli selection - (reply: 1)
823. orientation of promoter into viral construct - (reply: 1)
824. problems with PCR confirmation of insert . HELP ! - (reply: 1)
825. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
826. Loss of promoter during ligation - (reply: 2)
827. Enzyme restriction - (reply: 8)
828. Colony PCR Problem!!!! - (reply: 5)
829. Removing unwanted sequence from pUC19 - (reply: 4)
830. transformation fails, bacteria grows in control plate - (reply: 6)
831. competentce cell, cloning - (reply: 2)
832. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
833. co-transformation - (reply: 3)
834. pcr clean-up fail? - (reply: 1)
835. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
836. sequential cloning of multiple PCR products - how to do? (reply: 3)
837. Cloning large gene - (reply: 3)
838. Glycosilated peptide's cDNA cloning - (reply: 3)
839. pWM91 cloning problem - (reply: 4)
840. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)